God declared, “I have given every green herb for meat and it was so”. This suggest the enormous health benefits in green vegetation especially Moringa oleifera. This study examined the trypanocidal activity of Moringa oleifera seed on rats infected with Trypanosomal bruceibrucei (T.b.b) and Trypanosomal congolense (T.c). The acute toxicity (LD₅₀) was carried out and the plant showed no apparent signs of toxicity. In-vitro trypanocidal activity of Moringa seed showed the highest activity at 10mg/ml within five minutes of incubation. The in-vivo study also showed inhibition in the group infected and treated whereas, there was a case of death recorded in the groups infected without treatment. The mean body weight of the infected rats was observed to decrease steadily although not statistically significant at P<0.05. Hematology revealed a significant decrease (p< 0.05) in PCV and Hb, an indication of anemia but was significantly increased in the treated groups. Furthermore, rats infected with T.b.b and T.c, revealed a significant increase in levels of AST, ALT and ALP in the untreated groups but were significantly decreased (p<0.05) in the treated groups. The phytochemical screening of samples revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, antraquinones, steroid, phenols, cardiac glycosides, carbohydrates and tannins. From the findings, the seed extract of M. oleifera could serve as adjuvant to folder of cattle and other ruminants as a curative measure against T. b.b and T. c. 

The foliage of Indian plants is rich in variegation and these plant products are now gaining significant value. The main purpose of studies is to focus on the properties of such medicinal plants so that they can be used for drug preparation for many untreated diseases of juice and herbal extracts of green leaves from Syzygium cumini and Moringa oleifera. This study shows the antibacterialbenefits during the phytochemical study, auxiliary metaboliteswere observed. These bioactive mixtures can beused to cure several diseases. Aqueous and ethanolic extraction took place as the leaves of both species, and the qualitative and quantitative phytochemical properties were then individually tested. The existence of phytochemicals, such as flavonoids, tannins, saponins, hydroanthraquinones, terpenes, cardiac glycosides, phlobatannins, phenolics, proteins, carbohydrates etc. was shown in the standard of phytochemical research. The results of the phytochemical quantitative test compare how many secondary metabolites are present in the plant leaf extracts like flavonoids, but for protein ethanolic extracts were found more than others i.e. 10.94 mg/mL. The DPPH study showed that Moringa oleifera and Syzygium cumini have demonstrated the highest antioxidant activity i.e. 83.81% through the blend of ethanolic extraction. Study proves the medicinal qualities of Indian plants in which presence of phytochemicals indicates nutritional and medicinal properties of both strains to cure various ailments. For example, the tannins extracts from Syzgium cumini have gastroprotective and anti-ulcerogenic effects. The demonstration of the phytochemical analysis proved that the Indian medicinal plants have medicinal qualities.

Piper cubeba pet ether extract or hexane extract in the form of oil can be used as active ingredient in different types of herbal formulations. Cubebin gives very good medicinal effect in various   formulations, if used in specific concentration. Hence quantitative analysis of cubebin in final formulation is essential.Three types of formulations viz. tablet, candy and shampoo were prepared in the laboratory. Piper cubeba  oil (PC oil) i.e. the extract of Piper cubeba with minimum 1.5% Cubebin was used as an active ingredient in  appropriate concentrations in all formulations. Vaginal tablet with 100mg PC oil per tablet, Candy with 9mg PC oil per candy and 2.5%v/v and 5%v/v PC oil shampoo were manufactured. Placebo of shampoo, PC oil sample were also analysed along individual lots of corresponding formulations. Many methods are available for determination of Cubebin. Attempts were made to apply the most common, easy, accurate, precise, and time saving High Performance Thin Layer Chromatography (HPTLC) for quantification of Cubebin in final formulations. Special care was taken while preparing the sample to avoid interference of sugar and excipients, in formulations like candy and shampoo.Findings of each formulation are found to be well within theoretically calculated specified range..Hence one can successfully apply HPTLC method for estimation of Cubebin in different formulations

Menstrual disorders are a major lifestyle issue in adolescent girls affecting their quality of life. In view of this, green tea (Camellia sinensis), a commonly consumed non-fermented beverage was developed, with an enrichment of bioactive ingredients like chamomile (Matricaria chamomilla), shatavari (Asparagus racemosus), moringa leaves (Moringa oleifera), cinnamon (Cinnamomum zeylanicum), ginger (Zingiber officinale Rosc), and fennel seeds (Foeniculum vulgare) and its micronutrient, tannin and antioxidant properties were evaluated. Green tea was used as the standard (T0) and variations were formulated with bioactive ingredients in varying amounts (V1, V2, V3). The most acceptable variation (V2) was considered for further evaluation. V2 was found to contain 14.1±1.66 mg/100g iron, 11.45±1.52 mg/100g vitamin C, and 13.5±0.5 mg/100 g zinc and 7.6±0.15 mg/100 g tannin. Higher antioxidant assay was found in V2 for total polyphenol content (1.39±0.29mg GAE/g) and DPPH radical scavenging activity with 142.6µg/ml of IC50 value. A nudge-based intervention was carried out for 4 weeks among adolescent girls to study the effectiveness of the consumption of enriched tea (V2) on the relief of menstrual disorder symptoms. A significant decrease (p<0.05) in the symptoms as well as the impact of those symptoms on their daily activities was observed between pre and post intervention. Hence the developed bioactive enriched green tea contains the ingredients rich in antioxidants and iron.">

Down syndrome is a genetic disorder caused by the presence of extra genetic material from chromosome in some or all of an individual’s cells. The condition reduces cognitive ability and results in certain characteristic physical features. This case report is aimed at dissemination of role of Ayurveda in management of Down syndrome. This case study of 2.4 years male presented with Down syndrome and global delay.  It has shown promising results. It opened the door of Ayurvedic approach with hope to deliver the good result in similar disorders

Guava leaves rich in micronutrient and bioactive compounds is a promising nutraceutical food which can be used as a potential ingredient to curtail the unabated rise of malnutrition and anemia. The objective of this study was the formulation of Iron Rich Mix Flour (IRMF) using underutilized guava leaves, evaluate its nutritional and microbial properties and study the sensory characteristics of IRMF incorporated Ready-To-Eat (RTE) products (cookies, khakhra and pizza base). IRMF had a rich nutrient profile of 298 kcals, 41.5g carbohydrate, 25.4 g protein, 2.3g fat, 10.5g crude fibre, 8.7 mg iron, 580.5mg calcium, 37.8mg vitamin C and low tannin content- 1.4g%. It had an excellent shelf life of 21 days. Half maximal inhibitory concentration (IC 50 value) was found to be less in the formulated IRMF which indicates potential radical scavenging activity thus exhibiting antioxidant activity. Organoleptic evaluation of RTE products was done by 25 panelists using 9-point hedonic scale. The sensory acceptance of IRMF at 0%, 40%, 50% and 60% into recipes were significant for all the 3 products (p<0.05). Results showed that khakhra with 50% IRMF incorporation obtained the highest overall acceptability (8.84 ± 0.01). The formulated products are therefore energy dense, carbohydrate and protein rich, providing a good amount of micronutrients. Successful incorporation of these ingredients with promising nutraceutical properties will pave new way for innovations in the snack food sector of food industry and help tackle public health issues of malnutrition and anemia. 

Infertility is vital medical and social problems in the world. 40% couples are infertile due to male factor infertility. Sexual dysfunction occurs in 10-52% of men. Male sexual dysfunctions (MSD) have been elaborately illustrated under the broad heading of Klaibya in Ayurvedic classics. Kapikacchu (Mucuna pruriens Linn., Family- Fabaceae) has several functions like to treat Parkinson’s disease, impotence, worms, diuretic, aphrodisiac, nerve tonic. It is a potential Vrishya (aphrodisiac) drug in Ayurveda. Its seeds enhance sexual drive and power while improving the quality of the sperm.  By the virtues of Madhura Rasa and Vipaka, Guru, Snigdha Guna, it performs the functions of Dhatuposhan, Shukravardhan and demonstrates Vrishya, shukrakara, vajikarana, Brihana and Rasayana actions. It provides higher impact on sexual parameters by alleviating the Vata Dosha that is the root cause in the manifestation of Klaibya. It has Sheeta Virya, so it delays ejaculation in case of premature ejaculation. It improves male fertility by its action on the hypothalamus-pituitary-gonadal axis. It reactivates the antioxidant defence system of infertile men moreover helps in the management of stress and improves semen quality, sperm count and sperm motility. Furthermore it significantly increases the sexual desire, penile rigidity, erection and duration of ejaculation with orgasm. The present review is an effort to highlight data about Ayurvedic aspect and relevant pharmacological studies for validation of vrishya (aphrodisiac) action of Kapikacchu. The interpretation of phyto-chemical and pharmacological study of this plant proves the submissions made in the Ayurvedic classics about its Vrishya i.e. aphrodisiac activity.